KB 사람 구강 편평 암세포에서 Lyophyllum shimeji로부터 분리한 단백질 추출물의 항암활성
Anti-cancer activity of the protein extract from Lyophyllum shimeji in KB human oral squamous cell carcinoma
정종원, 문성민, 박보람, 김수관, 김진수, 김춘성,
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정종원 ( Jung Jong-Won ) - 조선대학교 치의학전문대학원 구강생물학연구소
문성민 ( Moon Sung-Min ) - 조선대학교 치의학전문대학원 구강생물학연구소
박보람 ( Park Bo-Ram ) - 조선대학교 치의학전문대학원 구강생물학연구소
김수관 ( Kim Su-Gwan ) - 조선대학교 치의학전문대학원 구강생물학연구소
김진수 ( Kim Jin-Soo ) - 조선대학교 치의학전문대학원 구강생물학연구소
김춘성 ( Kim Chun-Sung ) - 조선대학교 치의학전문대학원 구강생물학연구소
KMID : 0353320140380020099
Abstract
Purpose: To explore its potential anti-cancer effect, we examined whether or not extract of Lyophyllum shimeji could inhibit growth of KB human oral squamous cell carcinoma and investigated its anti-cancer mechanism.
Materials and Methods: This study examined the anti-effects of protein extract from L. shimeji as well as its molecular mechanism of action on KB human oral squamous cell carcinoma. To determine whether or its mechanism is related to apoptosis, we performed DNA fragmentation, Western blotting, and LIVE-DEAD cell staining.
Results: Treatment with extract from L. shimeji reduced cell viability of KB oral squamous cell carcinoma in a dose-dependent manner. Formation of a DNA ladder was observed by treatment with extract from L. shimeji. Western blot analysis showed that extract from L. shimeji induced activation of cleaved caspases-7 and -9 as well as cleaved poly-ADP-ribose polymerase. Furthermore, we confirmed that dead cell staining (red color) was significantly increased by extract from L. shimeji.
Conclusion: L. shimeji exhibited cytotoxicity on KB humans oral squamous cell carcinoma, and its effect was mainly mediated by apoptosis. Thus, L. shimeji could be considered as a potential safe and effective chemotherapy agent for the treatment of oral cancer. In addition, these results suggest that extract from L. shimeji induces cell apoptosis through a mitochondrial caspase-dependent pathway in KB humans oral squamous cell carcinoma.
키워드
Apoptosis; Oral cancer; Lyophyllum shimeji
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